Preparation for scRNA-seq analysis
Important
You will need to review this material before class 31.
Experimental methods
In this section we will analyze data generated by the 10x Genomics Chromium scRNA-seq platform. The following paper introduces the technology:
Zheng GXY, Terry JM, Belgrader P, et al. Massively parallel digital transcriptional profiling of single cells. Nat Communications. 2017;8:ncomms14049. https://doi.org/10.1038/ncomms14049 [Link].